Isolation of NADPH:isoflavone oxidoreductase, a new enzyme of Pterocarpan phytoalexin biosynthesis in cell suspension cultures of Cicer arietinum

@inproceedings{Tiemann1987IsolationON,
  title={Isolation of NADPH:isoflavone oxidoreductase, a new enzyme of Pterocarpan phytoalexin biosynthesis in cell suspension cultures of Cicer arietinum},
  author={Karin Tiemann and Walter Hinderer and Wolfgang Barz},
  year={1987}
}
Chickpea Cicer arietinum L.) cell suspension cultures transferred into a medium containing yeast extract accumulate the phytoalexins medicarpin and maackiain. Concomitant with accumulation of the pterocarpans a new enzyme activity is induced which was characterized as NADPH:isoflavone oxidoreductase. Maximum enzyme activity was reached 16 h after transfer of cells and then activity rapidly declined. The soluble enzyme was partially purified and shown to catalyze the reduction of the isoflavone… CONTINUE READING