Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation

  title={Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation},
  author={Ying Liu and Keqiang Chen and Chunyan Wang and Wang-hua Gong and Teizo Yoshimura and Ji Ming Wang and Mingyong Liu},
[Abstract] A hallmark of cancer-associated inflammation is the infiltration of leukocytes into tumors, which is believed to be recruited by chemokines. Some infiltrating leukocytes such as macrophages often promote tumor growth by producing growth-inducing and angiogenic factors. Here, we described a method of isolating tumor-infiltrating leukocytes with Percoll density gradient, because Percoll possesses a low viscosity, a low osmolarity and no toxicity to cells. Different leukocyte… 

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