Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation

@inproceedings{Liu2013IsolationOM,
  title={Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation},
  author={Ying Liu and Keqiang Chen and Chunyan Wang and Wang-hua Gong and Teizo Yoshimura and Ji Ming Wang and Mingyong Liu},
  year={2013}
}
[Abstract] A hallmark of cancer-associated inflammation is the infiltration of leukocytes into tumors, which is believed to be recruited by chemokines. Some infiltrating leukocytes such as macrophages often promote tumor growth by producing growth-inducing and angiogenic factors. Here, we described a method of isolating tumor-infiltrating leukocytes with Percoll density gradient, because Percoll possesses a low viscosity, a low osmolarity and no toxicity to cells. Different leukocyte… 

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References

Cell surface receptor FPR2 promotes antitumor host defense by limiting M2 polarization of macrophages.

The results suggest that Fpr2 plays an important role in host defense against implanted LLC by sustaining macrophages in an M1 phenotype with more potent antitumor activities.