• Medicine
  • Published 2014

Isolation of Mouse Primary Hepatocytes by Retrograde Liver Perfusion with Catheterization via Heart

@inproceedings{Zhaoya2014IsolationOM,
  title={Isolation of Mouse Primary Hepatocytes by Retrograde Liver Perfusion with Catheterization via Heart},
  author={Yu Zhao-ya},
  year={2014}
}
Objective To explore the effects of retrograde liver perfusion with catheterization via heart on the isolation of primary mouse hepatocytes.Methods In order to more efficiently isolate primary mouse hepatocytes, we improved the traditional two-step collagenase perfusion method.The liver perfusion catheter was inserted through right atrium and suprahepatic vena cava,and the perfusion velocity was controlled by the drip infusion with collagenase perfusate containing 10% of fetal calf serum… CONTINUE READING

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