Isolation of Legionella-containing vacuoles by immuno-magnetic separation.

Abstract

The environmental bacterium Legionella pneumophila naturally parasitizes free-living amoebae. L. pneumophila is an opportunistic human pathogen that grows in macrophages, thus causing a life-threatening pneumonia termed Legionnaires' disease. The bacteria replicate intracellularly in environmental and immune phagocytes within a unique compartment, the… (More)
DOI: 10.1002/0471143030.cb0334s46

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