Isolation of Japanese encephalitis virus from Culex annulirostris in Australia.

  title={Isolation of Japanese encephalitis virus from Culex annulirostris in Australia.},
  author={Scott A. Ritchie and D. Phillips and Annette K. Broom and John Mackenzie and Michael Poidinger and Andrew F van den Hurk},
  journal={The American journal of tropical medicine and hygiene},
  volume={56 1},
Mosquitoes were collected using CO2 and octenol-baited light traps during an outbreak of Japanese encephalitis (JE) on Badu Island in the Torres Strait, Australia in April 1995. A total of 13,300 mosquitoes comprising 12 species were processed for virus isolation. Eight isolates of JE virus were obtained from Culex annulirostris, with a carriage rate of 2.97:1,000; this mosquito also yielded one Sindbis virus isolate. A reverse transcriptase-polymerase chain reaction was used to sequence the JE… CONTINUE READING


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