Isolation of Infectious Poliovirus Ribonucleic Acid from Supernatant Fluids after Ultracentrifugation

Huppert and Sanders1 reported earlier that after pelleting the intact encephalomyocarditis (EMC) virus in the ultracentrifuge and extracting both the pellet and supernatant with cold phenol, infectious ribonucleic acid (RNA) was obtained only from the supernatant. Recently, Colter et al.2 contradicted the above results; they found the yield of infectious… CONTINUE READING