Isolation of DNA sequences amplified at chromosome 19q13.1-q13.2 including the AKT2 locus in human pancreatic cancer.

@article{Miwa1996IsolationOD,
  title={Isolation of DNA sequences amplified at chromosome 19q13.1-q13.2 including the AKT2 locus in human pancreatic cancer.},
  author={Wataru Miwa and Jun Yasuda and Yuko Murakami and Kazuo Yashima and Kokichi Sugano and Takashi Sekine and Akira Kono and Shin‐ichi Egawa and Kazuhisa Yamaguchi and Yoshihide Hayashizaki and Takao Sekiya},
  journal={Biochemical and biophysical research communications},
  year={1996},
  volume={225 3},
  pages={968-74}
}
In the human pancreatic cancer cell line PANC1, we detected several DNA fragments with abnormally intensified signals by restriction landmark genomic scanning. Major five of these fragments were cloned. All of the cloned fragments were mapped at the 19q13.1-13.2 region where the AKT2 oncogene was located. Southern blotting using the cloned DNA fragments and a fragment of AKT2 cDNA as probes revealed that the AKT2 gene was amplified in 3 of 12 pancreatic cancer cell lines analyzed including… CONTINUE READING
38 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 38 extracted citations

Similar Papers

Loading similar papers…