Isolation of Commensal Bacteria from Umbilical Cord Blood of Healthy Neonates Born by Cesarean Section

@article{Jimnez2005IsolationOC,
  title={Isolation of Commensal Bacteria from Umbilical Cord Blood of Healthy Neonates Born by Cesarean Section},
  author={Esther Jim{\'e}nez and Le{\'o}nides Fern{\'a}ndez and Mar{\'i}a L. Mar{\'i}n and Rocío Martín and Juan Manuel Odriozola and Carmen Nueno-Palop and Arjan Narbad and M{\'o}nica Olivares and Jordi Xaus and Juan Miguel Rodr{\'i}guez},
  journal={Current Microbiology},
  year={2005},
  volume={51},
  pages={270-274}
}
In a previous study, lactic acid bacteria were isolated from meconium obtained from healthy neonates born by cesarean section. Such a finding suggested that term fetuses are not completely sterile, and that a mother-to-child efflux of commensal bacteria may exist. Therefore, presence of such bacteria in umbilical cord blood of healthy neonates born by elective cesarean section was investigated. The blood samples were submitted to an enrichment step and then inoculated onto agar plates. All the… 

Figures and Tables from this paper

Is meconium from healthy newborns actually sterile?
Sensitive Quantitative Analysis of the Meconium Bacterial Microbiota in Healthy Term Infants Born Vaginally or by Cesarean Section
TLDR
Lower detection rate of lactobacilli in cesarean-born babies suggests that the primary source of lacto-gut bacteria in infant gut is mainly from maternal vaginal and–to a lesser extent–anal microbiota during vaginal delivery, and that the colonization by some important Lactobacillus species is delayed in babies delivered via cesar-section.
Preterm infant gut colonization in the neonatal ICU and complete restoration 2 years later.
  • L. Moles, M. Gómez, R. del Campo
  • Medicine
    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
  • 2015
Antenatal Microbial Colonization of Mammalian Gut
TLDR
A possible antenatal colonization of the developing mammalian gut is suggested by analyzing sterilely collected intestinal tissues, placenta, and amniotic fluid from rodent fetuses and tissues from autoptic human fetuses to confirm the high interindividual variability of commensal microbiota even in the antenatal period.
Extremely preterm neonates have more Lactobacillus in meconium than very preterm neonates – the in utero microbial colonization hypothesis
TLDR
The hypothesis that maternal bacteria from the gut and vagina can play a role in shaping neonates’ gut microbiota and that mother-to-infant bacterial transmission is a controlled and time-specific process is supported.
Parallel detection of lactobacillus and bacterial vaginosis-associated bacterial DNA in the chorioamnion and vagina of pregnant women at term
  • Sophia M. R. Lannon, K. A. Adams Waldorf, D. Fredricks
  • Medicine, Biology
    The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
  • 2019
TLDR
Microbial trafficking of lactobacilli and fastidious bacteria into the chorioamniotic membranes and choriodecidua occurs at term in normal pregnancies and in one case, a quantitative gradient between multiple bacterial species in the lower genital tract and placenta is demonstrated.
Evidence for contamination as the origin for bacteria found in human placenta rather than a microbiota
TLDR
It is shown that the isolation of meaningful quantities of viable bacteria or bacterial DNA was possible only outside the placenta, highlighting the importance of sampling methods in studying the in utero environment.
First-Pass Meconium Samples from Healthy Term Vaginally-Delivered Neonates: An Analysis of the Microbiota
TLDR
Evidence is provided that low numbers of bacteria are present in first-pass meconium samples from healthy, vaginally-delivered, breastfed term infants, suggesting that gut bacterial colonisation is extremely limited at birth and occurs rapidly thereafter.
Streptococcus mutans in Umbilical Cord Blood, Peripheral Blood, and Saliva from Healthy Mothers
TLDR
The majority of children can have contact with SM-DNA during the intrauterine life by the CB, probably transferred from salivary habitat to PB and CB, and the tooth brushing can be associated to S. mutans detection in blood samples.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 25 REFERENCES
Molecular epidemiological study of vertical transmission of vaginal Lactobacillus species from mothers to newborn infants in Japanese, by arbitrarily primed polymerase chain reaction
TLDR
It is suggested that approximately one-fourth of infants acquire vaginal lactobacilli from their mothers at birth, and that the acquired lactobACilli do not last in the intestine of the infant long-term, but rather, are replaced by ones from milk or unknown sources after birth.
Human milk is a source of lactic acid bacteria for the infant gut.
Molecular Monitoring of Succession of Bacterial Communities in Human Neonates
TLDR
This study showed that using PCR-DGGE and 16S rDNA sequence analysis together resulted in a dynamic description of bacterial colonization in the infant intestinal ecosystem and allowed visualization of bacteria that are difficult to cultivate or to detect by other methods.
Broad-spectrum bacterial rDNA polymerase chain reaction assay for detecting amniotic fluid infection among women in premature labor.
  • J. Hitti, D. Riley, D. Eschenbach
  • Medicine, Biology
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
  • 1997
TLDR
The broad-spectrum bacterial 16S rDNA PCR assay may prove useful for diagnosing amniotic fluid infection and may be underestimated on the basis of amniotics fluid culture results.
Natural History of Streptococcus sanguinis in the Oral Cavity of Infants: Evidence for a Discrete Window of Infectivity
TLDR
It is found that the colonization of S. sanguinis occurs during a discrete “window of infectivity” at a median age of 9 months in the infants, and this in turn may suggest several ecological approaches toward controlling dental caries.
Salivary Actinomyces naeslundii genospecies 2 and Lactobacillus casei levels predict pregnancy outcomes.
TLDR
It is concluded that other oral bacterial species can also be related to pregnancy outcomes in addition to previously reported periodontal pathogens.
Developmental microbial ecology of the neonatal gastrointestinal tract.
TLDR
In this review, the development of the intestinal microbiota is discussed in terms of initial acquisition and subsequent succession of bacteria in human infants and the advantages of modern molecular ecology techniques that provide sensitive and specific, culture-independent evaluation of the gastrointestinal ecosystem are introduced.
H(2)O(2) produced by viridans group streptococci may contribute to inhibition of methicillin-resistant Staphylococcus aureus colonization of oral cavities in newborns.
  • Y. Uehara, K. Kikuchi, K. Totsuka
  • Medicine, Biology
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
  • 2001
TLDR
Results indicate that H( 2)O(2) produced by viridans group streptococci may inhibit MRSA colonization of oral cavities in newborns and prevent methicillin-resistant Staphylococcus aureus colonization of the oral cavity of newborns.
The subgingival microbial flora during pregnancy.
TLDR
Pregnancy and specifically steroid hormones appear capable of influencing the normal bacterial flora and inducing alterations in the subgingival ecology.
...
1
2
3
...