Isolation of Chromoplasts and Suborganellar Compartments from Tomato and Bell Pepper Fruit.

Tomato is a model for fruit development and ripening. The isolation of intact plastids from this organism is therefore important for metabolic and proteomic analyses. Pepper, a species from the same family, is also of interest since it allows isolation of intact chromoplasts in large amounts. Here, we provide a detailed protocol for the isolation of tomato… CONTINUE READING