Isolation of Agrobacterium sp. strain KNK712 that produces N-carbamyl-D-amino acid amidohydrolase, cloning of the gene for this enzyme, and properties of the enzyme.

Abstract

Agrobacterium sp. strain KNK712, which produced N-carbamyl-D-amino acid amidohydrolase (DCase) was isolated from soil. The bacterium had D-specific hydantoinase activity also. Both enzymes are suitable for use in the production of D-amino acids. The DCase gene from Agrobacterium sp. strain KNK712 was cloned into Escherichia coli. The cloned DNA fragment… (More)

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@article{Nanba1998IsolationOA, title={Isolation of Agrobacterium sp. strain KNK712 that produces N-carbamyl-D-amino acid amidohydrolase, cloning of the gene for this enzyme, and properties of the enzyme.}, author={Hirokazu Nanba and Yasuhiro Ikenaka and Y. Yamada and Kazuyoshi Yajima and M. Takano and S. Takahashi}, journal={Bioscience, biotechnology, and biochemistry}, year={1998}, volume={62 5}, pages={875-81} }