Isolation from human cerebrospinal fluid of a new insulin‐like growth factor‐binding protein with a selective affinity for IGF‐II

@article{Roghani1989IsolationFH,
  title={Isolation from human cerebrospinal fluid of a new insulin‐like growth factor‐binding protein with a selective affinity for IGF‐II},
  author={Monireh Roghani and Paul Hossenlopp and Pierre Lepage and Alain Balland and Michel Binoux},
  journal={FEBS Letters},
  year={1989},
  volume={255}
}
Two insulin-like growth factor (IGF)-binding proteins are responsible for the selective affinity for IGF-II of cerebrospinal fluid binding proteins.
TLDR
The affinity of the 32-30K BP is the strongest among the BPs identified to date, and it would seem that IGFBP-1 has two classes of IGF-binding site, one of high and one of low (less than 10(9) M-1) affinity for both IGFs.
Isolation and molecular cloning of insulin-like growth factor-binding protein-6.
TLDR
A comprehensive purification scheme using gel filtration, ligand-affinity chromatography, and several steps of reverse phase HPLC to isolate all the BPs in pig ovarian follicular fluid yielded five distinct IGFBPs, and upon analysis, they were found to correspond to the previously identified human and rat IGFBP-2, -3, -4, -5, and -6.
Insulin-like growth factor binding proteins in cerebrospinal fluid during human development and aging.
TLDR
Results show that IGFBP-2, 3, 4 and 5 in CSF are developmentally regulated, suggesting roles for these molecules in the development of the nervous system.
Radioimmunoassay of insulin-like growth factor-binding protein-6 in human serum and other body fluids.
TLDR
A radioimmunoassay has been established for the insulin-like growth factor-binding protein, IGFBP-6, isolated from a human transformed fibroblast cell-line, and its role as an IGF carrier is facilitated.
Characterization of insulin-like growth factor binding proteins produced in the rat central nervous system.
TLDR
CSF contains a mixture of distinct IGFBPs, primarily IGFBP-2, which are capable of being synthesized locally within the central nervous system by glial cells and neurons, suggesting that they are not derived from plasma by transport across the blood-brain barrier.
Hormonal regulation of insulin-like growth factor binding protein-1 expression in the rat.
TLDR
Although the exact number of these binding proteins present in serum remains to be determined, evidence for the existence of at least five distinct binding proteins has been presented in the recent literature.
Insulin-like growth factor (IGF)-I,-II and IGF-binding proteins in the cyst fluid of a patient with astrocytoma
TLDR
Insulin-like growth factor (IGF)-I,II and IGF-binding proteins (IGFBPs) were demonstrated in the cyst fluid of a patient with a hypothalamic astrocytoma, suggesting that IGFs as well as IGF- binding proteins are produced by astroCytoma cells and may act in a paracrine or autocrine fashion capable of modulating the growth of astro Cytoma tumours.
Characterization of a low molecular mass form of insulin-like growth factor binding protein-3 (17.7 kilodaltons) in urine and serum from healthy children and growth hormone (GH)-deficient patients: relationship with GH therapy.
TLDR
The objectives of this study were to characterize the molecular weight forms of IGFBP-3 in urine and serum of healthy children and adults and in children with GH deficiency (GHD), to quantify the urinary molecular weight Forms of IGF BP-3, and to evaluate the relationship of these forms with GH therapy.
Purification of a colon cancer cell growth inhibitor and its identification as an insulin-like growth factor binding protein.
TLDR
NH2-terminal amino acid sequence analysis of the first 32 residues has demonstrated that this protein belongs to the insulin-like growth factor-binding protein (IGFBP) family, and appeared to be identical to the recently cloned human IGFBP-4.
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TLDR
It is concluded that the 28K IGF-binding protein from amniotic fluid, like the previously purified 53K binding protein, has high affinity for both IGF-I and IGF-II, that it coexists in amniotics fluid with BP-53 or a related protein, and that the levels of both proteins decline with increasing fetal maturity.
Insulin-like growth factor-binding protein from human plasma. Purification and characterization.
TLDR
Competitive binding curves using pure BP and human IGF-I and IGF-II as both labeled and unlabeled ligands indicated association constants of 2-3 X 10(10) liters/mol for both peptides, with a slightly higher affinity for IGF- II than IGF- I, and 0.9 binding sites for either peptide per 53-kDa protein.
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TLDR
The absence of high-Mr IGF-BP complexes in the liver culture media, and yet the presence of the 41,500 and 38,500-Mr forms, which are the only binding units of the serum 'large' complex, indicates that these two binding proteins are capable of binding IGFs to form 'monomeric' IGF- BP complexes.
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Amniotic fluid binding protein (AFBP) is a heat and acid stable somatomedin (Sm)-binding protein with a mol wt of 35-40,000 and an isoelectric point of +/- 4.7. It is reactive in RRAs for Sm and
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TLDR
The placenta radioreceptorassay for somatomedin can be used for detection of the binding protein, which has a high content of acidic/amidated residues and was isolated from human amniotic fluid by a three-step procedure.
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TLDR
Competitive binding studies on binding proteins separated by chromatofocusing or by SDS-PAGE and transfer onto nitrocellulose revealed different affinities for the IGFs among the different molecular forms.
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