Isolation from genomic DNA of sequences binding specific regulatory proteins by the acceleration of protein electrophoretic mobility upon DNA binding.

@article{Subrahmanyam1999IsolationFG,
  title={Isolation from genomic DNA of sequences binding specific regulatory proteins by the acceleration of protein electrophoretic mobility upon DNA binding.},
  author={Satyam Subrahmanyam and John E. Cronan},
  journal={Gene},
  year={1999},
  volume={226 2},
  pages={263-71}
}
We report an efficient and flexible in vitro method for the isolation of genomic DNA sequences that are the binding targets of a given DNA binding protein. This method takes advantage of the fact that binding of a protein to a DNA molecule generally increases the rate of migration of the protein in nondenaturing gel electrophoresis. By the use of a… CONTINUE READING