Isolation from alfalfa of resistance gene analogues containing nucleotide binding sites

  title={Isolation from alfalfa of resistance gene analogues containing nucleotide binding sites},
  author={Julio Garc{\'i}a ‐ Cordero and D. Skinner},
  journal={Theoretical and Applied Genetics},
Cloned resistance (R) genes from a broad range of plant species are known to share similarities in DNA sequence and structural motifs. Degenerate oligonucleotide primers designed from conserved regions of the nucleotide binding site (NBS), common to many R genes, were used to amplify the NBS regions from genomic DNA from alfalfa (Medicago sativa L). Sequence comparisons of the amplified fragments indicated that at least 18 families of NBS-containing R genes are present in alfalfa. Comparisons… CONTINUE READING
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