Isolation and structure of mycobactin T, a growth factor from Mycobacterium tuberculosis.

  title={Isolation and structure of mycobactin T, a growth factor from Mycobacterium tuberculosis.},
  author={Glenn A. Snow},
  journal={The Biochemical journal},
  volume={97 1},
  • G. Snow
  • Published 1965
  • Biology, Medicine
  • The Biochemical journal
1. A growth factor for Mycobacterium johnei has been isolated from Mycobacterium tuberculosis as an iron complex; it has been named ferric mycobactin T. 2. Ferric mycobactin T as isolated is a mixture of at least four closely related components differing only in their fatty acid side chains. 3. Mycobactin T resembles mycobactin P in structure but differs in the following respects: (a) the aromatic acid residue is salicylic acid in place of 2-hydroxy-6-methylbenzoic acid; (b) the hydroxy acid… Expand
Structure of mycobactin J
Mycobactin J-1, an iron chelate fromMycobacterium paratuberculosis, was characterized by mass spectrum and by1H nuclear magnetic resonance (NMR) and13C NMR spectra of the parent molecule and ofExpand
A covalent adduct of MbtN, an acyl-ACP dehydrogenase from Mycobacterium tuberculosis, reveals an unusual acyl-binding pocket.
St steady-state kinetics show that MbtN has ACAD activity, preferring acyl chains of at least 16 C atoms, and the acyl-binding pocket adopts a different orientation to other structurally characterized ACADs, correlated with the apparent ability of MBTN to catalyse the formation of an unusual cis double bond in the mycobactin acyl chain. Expand
Mycobactins and exochelins of Mycobacterium tuberculosis, M. bovis, M. africanum and other related species.
Following iron-deficient growth, mycobactins and exochelins were isolated from 11 strains of Mycobacterium tuberculosis as well as from M. bovis and M. africanum, supporting previous claims that this group is a single taxon. Expand
Salicylic acid biosynthesis and its control in Mycobacterium smegmatis.
Synthesis of salicylic acid has been studied in washed cell suspensions and cell-free extracts of Mycobacterium smegmatis grown under iron-deficient conditions and the trivial name salicylate synthetase is proposed for the enzyme catalysing the final step from isochorismate. Expand
Iron transport in Mycobacterium smegmatis: the role of mycobactin.
A mechanism whereby mycobactin operates as a carrier for iron across the lipid envelope of theMycobacteria is described, and both compounds will be at their highest concentrations at the two lipid-water interfaces of the cell envelope: the outer and inner surfaces. Expand
Inhibition Studies of Mycobacterium tuberculosis Salicylate Synthase (MbtI)
This paper describes the first inhibition study of M. tuberculosis MbtI using a library of functionalized benzoate‐based inhibitors designed to mimic the substrate (chorismate) and intermediate (isochorismates) of the MBTI‐catalyzed reaction. Expand
Isolation, identification, and structural analysis of the mycobactins of Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, and Mycobacterium paratuberculosis
The findings indicate that there is a close taxonomic relationship between M. paratuberculosis and the MAIS complex and that these mycobactins were unique in that they had more than one alkyl chain. Expand
Analyses of MbtB, MbtE, and MbtF Suggest Revisions to the Mycobactin Biosynthesis Pathway in Mycobacterium tuberculosis
The results suggest revisions to the MBT biosynthesis pathway while also identifying new targets for antituberculosis drug development and suggest that the proposed tailoring enzymes are actually involved in precursor biosynthesis. Expand
This chapter discusses the biosynthesis and mechanism of action of hydroxamate-type siderochromes, and the role ofhydroxamic acids in iron transport by other organisms. Expand
A History of Iron Metabolism in the Mycobacteria
The review focuses mainly on the research work carried out over the last century leaving the present work on iron uptake to be covered in other reviews in this monograph. Expand