Isolation and serial cultivation of rabbit skin epithelial cells.

@article{Liu1978IsolationAS,
  title={Isolation and serial cultivation of rabbit skin epithelial cells.},
  author={S C Liu and Michal Kar{\'a}sek},
  journal={The Journal of investigative dermatology},
  year={1978},
  volume={70 5},
  pages={288-93}
}
A method to isolate and to serially cultivate rabbit skin epithelial cells from adult trunk skin has been developed. Using a collagen gel as substrate and trypsin and EDTA to dissociate cells, nonproliferative primary cultures of rabbit cells may be converted to proliferative populations, and at least 3 serial passages achieved. In the presence of large concentrations of methotrexate (up to 1000 microgram/ml), epithelial cells in primary culture show no decrease in their ability to attach… CONTINUE READING