Isolation and sequences of rice sucrose synthase cDNA and genomic DNA

  title={Isolation and sequences of rice sucrose synthase cDNA and genomic DNA},
  author={W Yu and A Wang and R Y Juang and Hsien-Yi Sung and Jong-Ching Su},
  journal={Plant Molecular Biology},
Genomic DNA and poly(A) + RNA were extracted and purified [1, 7] from excised leaflets and roots of 7to 10-day old etiolated seedlings and milk-ripe grains, respectively, of rice (Oryza sativa cv. Tainong 67). Genomic and cDNA libraries were constructed by ligating Sau 3A digest [2] and synthesized cDNA [3] on the Bam HI site of 2EMBL3 and the Eco RI site of 2gtl0 or pUC18 vectors, respectively [4, 7]. Screening of libraries was done by using a synthetic 17-mer (5'-TACATCTTCTTGAACTC-3', a… CONTINUE READING


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Publications referenced by this paper.
Showing 1-10 of 10 references

Different expression of two types of sucrose synthase-encoding genes in wheat in response to anaerobiosis, cold shock and light

  • C Marafia, F Garcia-Olmedo, P Carbonero
  • Gene 88:167-172
  • 1990
1 Excerpt

DNA sequence of a shrunken allele of maize: Evidence for visitation by insertional sequence

  • CD Zack, RJ Ferl, LC Hannah
  • Maydica 31:5-16
  • 1986

Techniques for transformation of E

  • D Hanahan
  • coll. In: Glover D (ed) DNA Cloning: A Practical…
  • 1985
1 Excerpt

A simple and very efficient method for generating cDNA libraries

  • U Gttbler, B JHoffman
  • Gene 25:263-269
  • 1983
1 Excerpt

Techniques for transformation of E . coll

  • D Hanahan
  • Gene
  • 1983

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