Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.

  title={Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.},
  author={Robert T. H. Ng and Jeanne Abelson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={77 7},
The yeast Saccharomyces cerevisiae is known to contain the highly conserved and unbiquitous protein actin. We have used cloned actin sequences from Dictyostelium discoideum to identify and clone the actin gene in yeast. Hybridization to genomic fragments of yeast DNA suggest that there is a single actin gene in yeast. We have determined the nucleotide sequence of that gene and its flanking regions. The sequence of the gene reveals an intervening sequence of 309 base pairs in the coding… CONTINUE READING
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