Isolation and sequence determination of two pyridoxal 5'-phosphate-labeled thermolysin peptides from pig muscle phosphoglucose isomerase.

Abstract

Pig muscle phosphoglucose isomerase modified with pyridoxal 5'-phosphate under conditions that cause at least 90% inactivation of its catalytic activity was found to incorporate about 1.5 eq of pyridoxal 5'-phosphate per subunit. After digestion with thermolysin, two pyridoxal 5'-phosphate-containing peptides were isolated and their amino acid sequences were determined to be Leu-Gly-pyridoxyl-Lys-Gln and Ile-Ala-Ser-pyridoxyl-Lys-Thr.

4 Figures and Tables

Statistics

0200400'86'89'92'95'98'01'04'07'10'13'16
Citations per Year

143 Citations

Semantic Scholar estimates that this publication has 143 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Palmieri1982IsolationAS, title={Isolation and sequence determination of two pyridoxal 5'-phosphate-labeled thermolysin peptides from pig muscle phosphoglucose isomerase.}, author={Richard H. Palmieri and D{\'o}nal Mc Gee and E A Noltmann}, journal={The Journal of biological chemistry}, year={1982}, volume={257 14}, pages={7965-8} }