Isolation and sequence analysis of canine respiratory coronavirus.

@article{Erles2007IsolationAS,
  title={Isolation and sequence analysis of canine respiratory coronavirus.},
  author={Kerstin Erles and Kai-Biu Shiu and Joe Brownlie},
  journal={Virus research},
  year={2007},
  volume={124 1-2},
  pages={78-87}
}
Canine respiratory coronavirus (CRCoV) has frequently been detected in respiratory samples from dogs by RT-PCR. In this report the first successful isolation of CRCoV from a dog with respiratory disease is described. The isolate CRCoV-4182 was cultured in HRT-18 cells but failed to replicate in a number of other cell lines. The nucleotide sequence of the 3'-terminal portion of the CRCoV genome was determined including all open reading frames from the NS2 gene to the N gene. Comparison with… CONTINUE READING
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