Isolation and reconstitution of the chloride transporter of clathrin-coated vesicles.

@article{Xie1989IsolationAR,
  title={Isolation and reconstitution of the chloride transporter of clathrin-coated vesicles.},
  author={Xiao-song Xie and Bill P Crider and D K Stone},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 32},
  pages={18870-3}
}
Clathrin-coated vesicle acidification is mediated by an endomembrane proton translocating ATPase. This pump is electrogenic, and significant pH gradient formation requires the parallel movement of chloride through a chloride transporter in order to maintain net electroneutrality. We have solubilized, isolated and achieved 270-fold purification of this chloride transporter by means of selective detergent solubilization with cholate and polyoxyethelene 9-lauryl ether (C12E9), hydroxylapatite… CONTINUE READING

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