Isolation and quantitation of HIV in peripheral blood.

  title={Isolation and quantitation of HIV in peripheral blood.},
  author={Richard A. Koup and David D. Ho and Guido Poli and Anthony S. Fauci},
  journal={Current protocols in immunology},
  volume={Chapter 12},
  pages={Unit 12.2}
Quantitation of replication-competent human immunodeficiency virus (HIV) in peripheral blood of infected individuals is critical for investigations of HIV pathogenesis and therapy. In this unit, the basic protocol determines the HIV titer in seropositive blood by measuring the tissue culture infectious dose (TCID) by an end-point dilution method. A second basic protocol utilizes the PHA-stimulated T cell blasts (activated T cells) in co-culture with PBMC as described in the first basic protocol… CONTINUE READING

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