Isolation and purification of poly(ADP-ribose) glycohydrolase from pig thymus.

@article{Tavassoli1983IsolationAP,
  title={Isolation and purification of poly(ADP-ribose) glycohydrolase from pig thymus.},
  author={Mehdi Tavassoli and Sydney Shall},
  journal={European journal of biochemistry},
  year={1983},
  volume={135 3},
  pages={449-55}
}
Poly(ADP-ribose) glycohydrolase has been purified about 12 300-fold from pig thymus with a recovery of 8.5%. The specific activity of the purified enzyme is 13.8 mumol min -1 mg protein -1. The molecular weight was estimated to be 59 000 by gel filtration through Sephadex G-100 in a non-denaturing solvent. Analysis of the final preparation by sodium dodecyl sulphate gel electrophoresis reveals two protein bands of molecular weight, 61 500 and 67 500. The Km value for poly(ADP-ribose) is… CONTINUE READING
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