Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography.

@article{Torruellas2005IsolationAP,
  title={Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography.},
  author={Carilyn Torruellas and Rong Tsao and Zhi-Qiang Liu and Shuying Liu and Raymond Yang and J. Christopher Young and Honghui Zhu and Ze-Yuan Deng and Mingyong Xie and Zhihong Fu},
  journal={Journal of chromatography. A},
  year={2005},
  volume={1063 1-2},
  pages={161-9}
}
Two isomeric phenylethanoid glycosides, acteoside and isoacteoside were isolated and purified from the seeds of Plantago psyllium L. for the first time by high-speed counter-current chromatography (HSCCC) using a solvent system consisting of ethyl acetate-water (1:1, v:v). By injecting 200 mg of the n-butanol extract of P. psyllium for five consecutive times, the two-step HSCCC procedure yielded a total of 165 mg of acteoside and 17.5 mg of isoacteoside from 978 mg extract. The recovery rates… CONTINUE READING

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