Isolation and properties of the DNA of African swine fever (ASF) virus.

@article{Enjuanes1976IsolationAP,
  title={Isolation and properties of the DNA of African swine fever (ASF) virus.},
  author={Luis Enjuanes and {\'A}ngel L. Carrascosa and E. Vinuela},
  journal={The Journal of general virology},
  year={1976},
  volume={32 3},
  pages={479-92}
}
African swine fever (ASF) virus was grown either in swine macrophages or in VERO cells and purified free of cell DNA. Virus DNA was isolated from virions as a molecule with a sedimentation coefficient of 60S and a contour of 58 +/- 3 mum. .these two values give a mol. wt. of 102 +/- 5 X 10(6) and 107 +/- 5 X 10(6), respectively, for the genome of ASF virus. Denatured DNA fragments from ASF virus reassociate with a C0t1/2 value of 0-60 +/- 0-05 MS, which compared with the corresponding value for… CONTINUE READING

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