Isolation and primary culture of viable multicellular endothelial isolates from hard corals

  title={Isolation and primary culture of viable multicellular endothelial isolates from hard corals},
  author={Elizabeth J. Kopecky and Gary K. Ostrander},
  journal={In Vitro Cellular & Developmental Biology - Animal},
Conditions for the primary culture of branching scleractinian coral (Acropora micropthalma and Pocillopora damicornis) cells were established with a calcium-free seawater cell dissociation method. Cells were isolated and cultured in supple-mented Dulbecco’s modified Eagle media with heat-inactivated fetal bovine serum, antibiotics, and sterile seawater. Among the isolated cell types, large (60–100 µm) multicellular endothelial isolates (MEIs) were seen in high numbers. These isolates were… CONTINUE READING
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