Isolation and phenotypic characterization of Chlamydomonas mutants defective in cytokinesis.

  title={Isolation and phenotypic characterization of Chlamydomonas mutants defective in cytokinesis.},
  author={Masafumi Hirono and Akinori Yoda},
  journal={Cell structure and function},
  volume={22 1},
Two genetically independent Chlamydomonas mutants, oca1 and oca2, that display abnormal cell division were isolated by DNA insertional mutagenesis. The culture of these mutants contained large abnormally-shaped cells with multiple pairs of flagella. DAPI staining showed that those aberrant cells carried the same number of nuclei as that of flagella pairs. Time-lapse video microscopy revealed the following characteristics of the cell division process in the mutants: i) although the mutants, like… CONTINUE READING

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