Isolation and partial characterization of two alcohol dehydrogenase isozymes from the medfly Ceratitis capitata.

@article{Gasperi1994IsolationAP,
  title={Isolation and partial characterization of two alcohol dehydrogenase isozymes from the medfly Ceratitis capitata.},
  author={Giuliano Gasperi and Dimitris Kafetzopoulos and Anna Christodoulidou and Vassilis Bouriotis and Charalambos Savakis},
  journal={Insect biochemistry and molecular biology},
  year={1994},
  volume={24 1},
  pages={87-94}
}
Two alcohol dehydrogenase isozymes, namely ADH-1 and ADH-2 from Ceratitis capitata were purified to homogeneity and further characterized. After ammonium sulphate precipitation from an extract of whole third instar larvae, the two isozymes were separated by ion exchange chromatography on Q-Sepharose. A combination of affinity chromatography, gel filtration and ion exchange chromatography was then used to purify each isozyme (50 and 57 times with 53 and 58% yields, for ADH-1 and -2 respectively… CONTINUE READING

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