Isolation and partial characterization of gamone 1 of Euplotes octocarinatus

  title={Isolation and partial characterization of gamone 1 of Euplotes octocarinatus},
  author={A. Weischer and Manfred Freiburg and Klaus Prof.Dr. Heckmann},
  journal={FEBS Letters},
The isolation of gamones 3 and 4 of Euplotes octocarinatus.
Gamones 3 and 4 of the ciliate Euplotes octocarinatus were isolated and purified to chromatographic and electrophoretic homogeneity to induce cells of certain mating types to unite in pairs and exchange gametic nuclei.
Gamone Production in Large-Scale Cultures of Euplotes octocarinatus1
Frequent exchange of culture fluid proved more effective than aeration in obtaining high cell densities and reasonable doubling times in large-scale cultures of the freshwater ciliate Euplotes octocarinatus.
Purification and initial characterization of two pheromones from the marine Antarctic ciliate, Euplotes nobilii
Two strains of wild‐type strains of Euplotes nobilii were found to be representative of different mating types mutually capable of inducing each other to form mating pairs through pheromones constitutively secreted into the extracellular environment.
Mating types and mating inducing substances in Euplotes octocarinatus
It is suggested that the transformation of the cells into cells ready for conjugation is triggered by the binding of gamone molecules to gamone-specific receptors and that mature cells usually expose receptors for all gamones except the ones they produce themselves.
Identification and characterization of a pheromone 2 specific binding protein of Euplotes octocarinatus
Heterologously-expressed pheromone 2 (Phr2) is used in affinity chromatography to isolate phermone 2 binding proteins in Euplotes octocarinatus and preliminary results indicate that antibodies raised against a C-terminal peptide of Phr2B inhibit cell mating.
Isolation of the Lembadion-Factor, a morphogenetically active signal, that induces Euplotes cells to change from their ovoid form into a larger lateral winged morph
The L-factor induces cells of the genus Euplotes to become less compact, which reduces their likelihood of becoming engulfed and develops extended wings and dorsal and ventral ridges and transforms within a few hours from its typical ovoid morph into an enlarged circular morph.


Blepharmone: A Conjugation-Inducing Glycoprotein in the Ciliate Blepharisma
Gamone 1 of Blepharisma intermedium was isolated, identified as a slightly basic glycoprotein (mizoleclular weight, 2 x 105), and designated as blepharmnone, which specifically transforms matinig type 2 cells, so that they can conjugate in about 2 hours.