Isolation and partial characterization of proteoglycans from rat incisors.

  title={Isolation and partial characterization of proteoglycans from rat incisors.},
  author={Firoz G Rahemtulla and Charles W. Prince and William T. Butler},
  journal={The Biochemical journal},
  volume={218 3},
Newly synthesized proteoglycans of rat incisors were labelled in vivo for 6h with [35S]-sulphate in order to facilitate their detection during purification and characterization. Proteoglycans were extracted from non-mineralized portions (predentine) of rat incisors with 4M-guanidinium chloride and subsequently from dentine by demineralization with a 0.4M-EDTA solution containing 4M-guanidinium chloride. Both extractions were performed at 4 degrees C in the presence of proteinase inhibitors… CONTINUE READING
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The Chemistry and Biology of Mineralized Connective Tissues

  • F. Rahemtulla, C. W. Prince, B. Caterson, J. E. Christner, J. R. Baker, W. T. Butler
  • (Veis, A.,
  • 1981
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