Isolation and morphology of calcium-tolerant feline ventricular myocytes.

@article{Silver1983IsolationAM,
  title={Isolation and morphology of calcium-tolerant feline ventricular myocytes.},
  author={Lewis H. Silver and E L Hemwall and Thomas A. Marino and Steven R. Houser},
  journal={The American journal of physiology},
  year={1983},
  volume={245 5 Pt 1},
  pages={H891-6}
}
A technique has been developed for isolating a high yield of Ca2+-tolerant rod-shaped myocytes from the right and left ventricles of cat myocardial tissue. Myocytes were prepared by retrograde perfusion of the coronary arteries via the aorta with a nominally Ca2+-free (20-30 microM) modified Krebs-Henseleit buffer containing 0.12% collagenase. After… CONTINUE READING