Isolation and molecular identification of free-living amoebae from dishcloths in Tenerife, Canary Islands, Spain

@article{ReyesBatlle2018IsolationAM,
  title={Isolation and molecular identification of free-living amoebae from dishcloths in Tenerife, Canary Islands, Spain},
  author={M. Reyes-Batlle and In{\'e}s Hern{\'a}ndez-Pi{\~n}ero and Aitor Rizo-Liendo and A. L{\'o}pez-Arencibia and I. Sifaoui and Carlos J. Bethencourt-Estrella and Olfa Chiboub and B. Valladares and J. Pi{\~n}ero and J. Lorenzo-Morales},
  journal={Parasitology Research},
  year={2018},
  volume={118},
  pages={927-933}
}
In this work, the presence of free-living amoebae (FLA) in dishcloths collected from human activity related places was evaluated. Once in the laboratory, 6 cm2 pieces of each dishcloth were cut and washed with Page’s Amoeba Solution (PAS) in sterile tubes. After washing, the dishcloth pieces were removed, and the tubes were centrifuged (1500 rpm for 10 min). The obtained pellets were seeded onto 2% non-nutrient agar (NNA) plates, incubated at room temperature and were monitored daily an… Expand
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