Isolation and molecular characterization of the [Fe]-hydrogenase from the unicellular green alga Chlorella fusca.

@article{Winkler2002IsolationAM,
  title={Isolation and molecular characterization of the [Fe]-hydrogenase from the unicellular green alga Chlorella fusca.},
  author={Martin Winkler and Burkhard Heil and Brian V. Heil and Thomas Happe},
  journal={Biochimica et biophysica acta},
  year={2002},
  volume={1576 3},
  pages={
          330-4
        }
}
[Fe]-hydrogenases are redoxenzymes that catalyze the reversible reduction of protons to hydrogen. Hydrogenase activity was observed in a culture of the unicellular green alga Chlorella fusca after an anaerobic incubation, but not in the related species Chlorella vulgaris. Specific polymerase chain reaction (PCR) techniques lead to the isolation of the cDNA and the genomic DNA of a special type of [Fe]-hydrogenase in C. fusca. The functional [Fe]-hydrogenase was purified to homogeneity and its N… CONTINUE READING
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