Isolation and molecular characterization of Candida africana from Jos, Nigeria.

@article{Nnadi2012IsolationAM,
  title={Isolation and molecular characterization of Candida africana from Jos, Nigeria.},
  author={Nnaemeka Emmanuel Nnadi and Grace Mebi Ayanbimpe and Fabio Scordino and Mark Ojogba Okolo and Ifeoma Bessy Enweani and Giuseppe Criseo and Orazio Romeo},
  journal={Medical mycology},
  year={2012},
  volume={50 7},
  pages={
          765-7
        }
}
During a survey of the prevalence of Candida spp. in Jos, Plateau State, Nigeria, two atypical C. albicans isolates were recovered. These two yeasts were germ tube positive, chlamydospore-negative and gave a green color on CHROMagar Candida. Molecular analysis performed by amplification of the hwp1 gene showed that these two isolates belonged to C. africana, a newly proposed Candida species closely related to C. albicans. Based on the presence or absence of an intron in DNA sequences encoding… 

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References

SHOWING 1-10 OF 32 REFERENCES

Candida africana and its closest relatives

It is found that C. africana has a worldwide distribution and a considerable number of features making its study particularly interesting, and the development of rapid and accurate tests for its discrimination from C. albicans, Candida dubliniensis and Candida stellatoidea is highlighted.

Candida africana sp. nov., a new human pathogen or a variant of Candida albicans?

This group of Candida strains isolated from patients in Madagascar, Angola and Germany are suggested to represent a new species within the genus Candida for which the name Candida africana is proposed.

Lack of Candida africana and Candida dubliniensis in Vaginal Candida albicans Isolates in Turkey Using HWP1 Gene Polymorphisms

The HWP1 gene polymorphisms offer a useful tool in the discrimination of C. africana and C. dubliniensis by using hyphal wall protein 1 (HWP1) gene polymorphism.

Candida dubliniensis sp. nov.: phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals.

Atypical oral Candida isolates recovered from 60 HIV-infected and three HIV-negative individuals strongly suggest that they constitute a novel species within the genus Candida for which the name Candida dubliniensis is proposed.

Phenotypic characterization of Candida Albicans from clinical sources in Nairobi, Kenya.

Non albicans candida are of clinical significance and may warrant the need to isolate and carry out phenotypic characterization Candida species from clinical setting and constantly monitor for fungal resistance as somecandida species are intrinsically resistant to certain antifungal drugs especially in the context of opportunistic infections in HIV/AIDS.

Prevalence of yeast among children in Nigeria and the United States.

The findings support previously reported observations that there may be intrinsic differences between different populations sampled and that malnutrition might favor the presence of yeast species other than C. albicans.

Molecular and Phenotypic Characterization of Genotypic Candida albicans Subgroups and Comparison withCandida dubliniensis and Candida stellatoidea

The results indicate that there is a correlation between the Candida groups and antifungal susceptibility and those strains previously designated C. albicansgenotype D are in fact C. dubliniensis.

Phenotypic and Molecular Characterization of Candida nivariensis sp. nov., a Possible New Opportunistic Fungus

The analyses of the internal transcribed spacer region and the D1-D2 region of the 26S rRNA gene sequences support a new species designation Candida nivariensis.

Candida dubliniensis: first identification in Sfax hospital, Tunisia

In this study, isolation and identification of C. dubliniensis from 12 human immunodeficiency virus‐negative patients hospitalised in the intensive care unit (ICU) at Habib Bourguiba Sfax Hospital is reported for the first time.

Candida bracarensis sp. nov., a novel anamorphic yeast species phenotypically similar to Candida glabrata.

Sequence analysis of the 26S rDNA D1/D2 gene variable region revealed that the two clinical isolates were closely related phylogenetically to C. glabrata and K. delphensis, but differed sufficiently to justify their assignment as representatives of a separate species.