Isolation and live imaging of enteric progenitors based on Sox10-Histone2BVenus transgene expression.

@article{Corpening2011IsolationAL,
  title={Isolation and live imaging of enteric progenitors based on Sox10-Histone2BVenus transgene expression.},
  author={Jennifer C. Corpening and Karen K Deal and Virginia Cantrell and Stephanie B. Skelton and Dennis P. Buehler and E. Michelle Southard-Smith},
  journal={Genesis},
  year={2011},
  volume={49 7},
  pages={599-618}
}
To facilitate dynamic imaging of neural crest (NC) lineages and discrimination of individual cells in the enteric nervous system (ENS) where close juxtaposition often complicates viewing, we generated a mouse BAC transgenic line that drives a Histone2BVenus (H2BVenus) reporter from Sox10 regulatory regions. This strategy does not alter the endogenous Sox10 locus and thus facilitates analysis of normal NC development. Our Sox10-H2BVenus BAC transgene exhibits temporal, spatial, and cell-type… CONTINUE READING
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