Isolation and in vitro culture of rare cancer stem cells from patient-derived xenografts of pancreatic ductal adenocarcinoma.

@article{Gach2013IsolationAI,
  title={Isolation and in vitro culture of rare cancer stem cells from patient-derived xenografts of pancreatic ductal adenocarcinoma.},
  author={Philip C Gach and Peter J Attayek and Gabriela S Herrera and Jen Jen J. Yeh and Nancy L Allbritton},
  journal={Analytical chemistry},
  year={2013},
  volume={85 15},
  pages={7271-8}
}
Described is the construction of a large array of releasable microstructures (micropallets) along with screening and isolation protocols for sorting rare, approximately 1 in 10,000, cancer stem cells (CSCs) from a heterogeneous cell population. A 10.1 × 7.1 cm array of micropallets (50 × 50 × 75 μm structures and 25 μm micropallet gap) was fabricated on a large glass substrate, providing an array of approximately 1.3 million releasable microstructures. Image analysis algorithms were developed… CONTINUE READING