Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings

@inproceedings{Igawa2009IsolationAI,
  title={Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings},
  author={Tomoko Igawa and Masayuki Fujiwara and Hirotaka Takahashi and Tatsuya Sawasaki and Yaeta Endo and Motoaki Seki and Kazuo Shinozaki and Yoichiro Fukao and Yuki Yanagawa},
  booktitle={Journal of experimental botany},
  year={2009}
}
The majority of proteins in eukaryotic cells are modified according to highly regulated mechanisms to fulfill specific functions and to achieve localization, stability, and transport. Protein ubiquitination is one of the major post-translational modifications occurring in eukaryotic cells. To obtain the proteomic dataset related to the ubiquitin (Ub)-dependent regulatory system in Arabidopsis, affinity purification with an anti-Ub antibody under native condition was performed. Using MS/MS… CONTINUE READING

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