Isolation and identification of cancer stem-like cells from murine melanoma cell lines.

@article{Dou2007IsolationAI,
  title={Isolation and identification of cancer stem-like cells from murine melanoma cell lines.},
  author={Jun Dou and Meng Fei Pan and Ping Wen and Yating Li and Quan Yong Tang and Lili Chu and Fengshu Zhao and Chuilian Jiang and Weihua Hu and Kai Hu and Ning Gu},
  journal={Cellular & molecular immunology},
  year={2007},
  volume={4 6},
  pages={467-72}
}
In current study, cancer stem-like cells in the murine melanoma B16F10 cells were investigated. CD phenotypes of the B16F10 cells were analyzed by flow cytometry, and the specific CD phenotype cells from the B16F10 cells were isolated by MACS. Then we used colony formation assay in soft agar media, the cell growth assay in serum-free culture media as well as the tumorigenicity investigation of the specific CD phenotype cells in C57BL/6 mice, respectively, to identify cancer stem-like cells in… CONTINUE READING

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