Isolation and identification of aptamers from defibrotide that act as thrombin antagonists in vitro.


This study was designed to isolate and identify aptamer sequences, acting as thrombin antagonists, from defibrotide, a single-stranded DNA fraction. Two different aptamers were identified: 5'-GGTTGGATTGGTTGG-3' and 5'-GGTTGGATCGGTTGG-3'. A third aptamer: 5'-GGATGGATCGGTTGG-3' was found in the PCR product from the double-stranded defibrotide precursor. All… (More)


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