Isolation and identification of a novel motility-inhibiting factor from goat cauda sperm plasma membrane.

@article{Dungdung2003IsolationAI,
  title={Isolation and identification of a novel motility-inhibiting factor from goat cauda sperm plasma membrane.},
  author={Sandhya R Dungdung and G. C. Majumder},
  journal={Cellular and molecular biology},
  year={2003},
  volume={49 3},
  pages={413-20}
}
A motility inhibiting factor (MIF) in sperm plasma membrane of mammalian spermatozoa (goat) has been demonstrated. This factor has been purified to apparent homogeneity by Sepharose-6B affinity chromatography and DEAE-cellulose ion-exchange chromatography. The molecular weight of the isolated factor has been estimated as 98 kDa by molecular sieving and analytical HPLC. SDS-polyacrylamide gel electrophoresis of MIF gave a single band of 100 kDa, indicating that the factor is a monomer. MIF is a… CONTINUE READING