Isolation and identification of a cDNA clone coding for an HLA-DR transplantation antigen alpha-chain.

@article{Gustafsson1982IsolationAI,
  title={Isolation and identification of a cDNA clone coding for an HLA-DR transplantation antigen alpha-chain.},
  author={Kenth Gustafsson and Park Police Bill and Dan Larhammar and Klas G Wiman and Lena Claesson and L Schenning and B Servenius and J. Sundelin and Lars Rask and Per A. Peterson},
  journal={Scandinavian journal of immunology},
  year={1982},
  volume={16 4},
  pages={303-8}
}
Membrane-bound mRNA was isolated from Raji cells and enriched for message coding for the HLA-DR transplantation antigen alpha-chain by sucrose gradient centrifugation. Double-stranded cDNA was constructed from this mRNA fraction, ligated to plasmid pBR322, and cloned into Escherichia coli. By hybrid selection, a plasmid, pDR-alpha-1, able to hybridize with mRNA coding for the HLA-DR alpha-chain was identified. From the nucleotide sequence of one end of the insert an amino acid sequence was… CONTINUE READING