Isolation and identification of N2-fixing microorganisms from the rhizosphere of Capparis spinosa (L.)

@article{Andrade2004IsolationAI,
  title={Isolation and identification of N2-fixing microorganisms from the rhizosphere of Capparis spinosa (L.)},
  author={Galdino Andrade and Eduardo N{\'e}stor Esteban and Leonardo Velasco and Maria J Lorite and Eulogio J. Bedmar},
  journal={Plant and Soil},
  year={2004},
  volume={197},
  pages={19-23}
}
Four bacterial strains, Pseudomonas stutzeri var. mendocina, Comamonas sp., Agrobacterium tumefaciens biovar. 2 and Sphingobacterium sp., isolated from the rhizosphere of wild-grown caper (Capparis spinosa L.) plants were able to fix N2 as shown by their growth in nitrogen-free medium and by the acetylene reduction test. P. stutzeri var. mendocina and Comamonas sp. contained DNA homologous to the Klebsiella pneumoniae M5a1 nifHDK genes. No hybridization was found with total DNA from either A… Expand

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