Isolation and genotyping of potentially pathogenic Acanthamoeba and Naegleria species from tap-water sources in Osaka, Japan

@article{Edagawa2009IsolationAG,
  title={Isolation and genotyping of potentially pathogenic Acanthamoeba and Naegleria species from tap-water sources in Osaka, Japan},
  author={Akiko Edagawa and Akio Kimura and Takako Kawabuchi-Kurata and Yasuhiro Kusuhara and Panagiotis Karanis},
  journal={Parasitology Research},
  year={2009},
  volume={105},
  pages={1109-1117}
}
Here, we carried out a survey to determine the prevalence of free-living amoebae (FLA) in tap-water sources from rivers and water treatment plants located in Osaka Prefecture, Japan. A total of 374 raw water samples were collected from 113 sampling points. The samples were filtrated and transferred to non-nutrient agar plates seeded with a heat-killed suspension of Escherichia coli and incubated for 2 to 7 days at 30°C or 42°C. The plates were examined by microscopy to morphologically identify… CONTINUE READING
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