Isolation and genotyping of Helicobacter pylori from untreated municipal wastewater.

@article{Lu2002IsolationAG,
  title={Isolation and genotyping of Helicobacter pylori from untreated municipal wastewater.},
  author={Yingzhi Lu and Thomas E. Redlinger and Raquel Avitia and Adriana Galindo and Karen E Goodman},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 3},
  pages={1436-9}
}
For this study, we isolated Helicobacter pylori from wastewater by a series of steps beginning with immunomagnetic separation and cell culture. After Gram staining and three standard microbial tests, the 16S rRNA sequences of a total of 23 out of 37 putative H. pylori isolates were verified by PCR. Eleven H. pylori isolates were genotyped and fell into four vacA classes: those with the vacA allelic variants s1a and m1, s1b and m1, s2 and m2, or s2 and m1. Most H. pylori isolates were of the… CONTINUE READING

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