Isolation and genome characterization of a novel duck Tembusu virus with a 74 nucleotide insertion in the 3' non-translated region.

Abstract

During investigations into the outbreak of duck Tembusu virus (DTMUV) infection in 2011 in China, a DTMUV strain (DTMUV-AH2011) was isolated from the affected ducks. The length of the genome of the DTMUV-AH2011 strain was found to be 11,064 nucleotides and to possess 10,278 nucleotides of one open reading frame (ORF), flanked by 94 nucleotides of the 5' non-translated region (NTR) and 692 nucleotides of the 3' NTR. In comparison with five fully sequenced TMUV genomes, the genome of DTMUV-AH2011 had a 74 nucleotide insertion in the 3' NTR. Comparison of the DTMUV-AH2011 fully deduced amino acid sequences with those of other Tembusu virus strains reported recently in China showed they had a highly conserved polyprotein precursor, sharing 98.9% amino acid identities, at least. The overall divergences of amino acid substitutions were randomly distributed among viral proteins except for the protein NS4B, the protein NS4B was unchanged. Knowledge of the biological characters of DTMUV and the potential role of the insertion in the 3' NTR in RNA replication will be useful for further studies of the mechanisms of virus replication and pathogenesis.

DOI: 10.1080/03079457.2015.1006167

Statistics

010002000201520162017
Citations per Year

269 Citations

Semantic Scholar estimates that this publication has 269 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Wang2015IsolationAG, title={Isolation and genome characterization of a novel duck Tembusu virus with a 74 nucleotide insertion in the 3' non-translated region.}, author={Jingman Wang and Weiting Liu and Gang Meng and Kangning Zhao and Jinyan Gu and Puyan Chen and Rui-bing Cao}, journal={Avian pathology : journal of the W.V.P.A}, year={2015}, volume={44 2}, pages={92-102} }