Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae.

@article{Kishida1996IsolationAG,
  title={Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae.},
  author={Masao Kishida and Michiyo Tokunaga and Yuichi Katayose and Hiroyuki Yajima and A Kawamura-Watabe and Fumio Hishinuma},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1996},
  volume={60 5},
  pages={798-801}
}
The linear double stranded DNA plasmid pGKL1 encodes the yeast killer toxin complex (Gunge et al., 1981) of which the killing mechanism is not understood. We isolated and characterized eight mutants in Saccharomyces cerevisiae that were insensitive to both the intracellularly expressed 28-kDa killer subunit and the native killer toxin complex. These mutations (iki1 through iki5) were all recessive, and classified into five complementation groups. The iki2 mutation was mapped to a position near… CONTINUE READING

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