Isolation and functional characterization of mutant ferrochelatases in Saccharomyces cerevisiae.

@article{Gra1996IsolationAF,
  title={Isolation and functional characterization of mutant ferrochelatases in Saccharomyces cerevisiae.},
  author={Monika G{\'o}ra and Agnieszka Chacinska and J Rytka Rytka and Rosine Labbe-Bois},
  journal={Biochimie},
  year={1996},
  volume={78 2},
  pages={144-52}
}
Ferrochelatase is a mitochondrial inner membrane-bound enzyme that catalyzes the incorporation of ferrous iron into protoporphyrin, the last step in protoheme biosynthesis. It is encoded by the HEM15 gene in the yeast Saccharomyces cerevisiae. Five hem15 mutants causing defective heme synthesis and protoporphyrin accumulation were investigated. The mutations were identified by sequencing the mutant hem15 alleles amplified in vitro from mutant genomic DNA. A single nucleotide change, causing an… CONTINUE READING