Isolation and functional characterization of Spirulina D6D gene promoter: role of a putative GntR transcription factor in transcriptional regulation of D6D gene expression.

@article{Subudhi2008IsolationAF,
  title={Isolation and functional characterization of Spirulina D6D gene promoter: role of a putative GntR transcription factor in transcriptional regulation of D6D gene expression.},
  author={Sanjukta Subudhi and Pavinee Kurdrid and Apiradee Hongsthong and Matura Sirijuntarut and Supapon Cheevadhanarak and Wanchern Potacharoen},
  journal={Biochemical and biophysical research communications},
  year={2008},
  volume={365 4},
  pages={
          643-9
        }
}
Delta6-Desaturase (D6D) is a key enzyme that catalyzes the synthesis of gamma-linolenic acid (GLA), an essential polyunsaturated fatty acid. We report here the isolation and first functional characterization of the D6D gene promoter from Spirulina platensis C1. Functional analysis of this isolated promoter showed that the Spirulina promoter was functional in Escherichia coli. Site-specific mutation studies demonstrated that the -10 sequence (TATAAT), located at -33bp relative to the translation… CONTINUE READING
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