Isolation and expression of rat 1,25-dihydroxyvitamin D3 receptor cDNA.

  title={Isolation and expression of rat 1,25-dihydroxyvitamin D3 receptor cDNA.},
  author={James K. Burmester and Nobuyo N. Maeda and Hector F DeLuca},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={85 4},
The cDNA for the 1,25-dihydroxyvitamin D3 receptor has been isolated by screening a rat kidney lambda gt11 expression library with a mixture of three previously identified anti-receptor monoclonal antibodies. Protein produced from this cDNA reacts with each of the three monoclonal antibodies but does not react with an antibody specific for the porcine receptor or an irrelevant antibody. In addition, the expressed protein specifically binds 1,25-dihydroxyvitamin D3 but does not bind estradiol… CONTINUE READING
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