Isolation and expression analysis of miR165a and REVOLUTA from Brassica species

  title={Isolation and expression analysis of miR165a and REVOLUTA from Brassica species},
  author={G. T. Prasanna Kumari and Karuna Kusumanjali and Prem Shankar Srivastava and Sandip Das},
  journal={Acta Physiologiae Plantarum},
The present study was performed to survey the nature and extent of sequence conservation and divergence of miR165a and its target REVOLUTA (REV), a HD-ZIP III family member from Brassica encompassing both diploid and allotetraploid genomes. Post-transcriptional gene silencing (PTGS)-based regulation in plant requires precise pairing of the miRNA–mRNA target. We identified length and sequence polymorphisms in pre-miR165a, although 21 nt mature sequence was largely invariant. One Single… CONTINUE READING
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Molecular cloning, a laboratory mannual, 3rd edn

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