Isolation and enzymic properties of levansucrase secreted by Acetobacter diazotrophicus SRT4, a bacterium associated with sugar cane.

@article{Hernandez1995IsolationAE,
  title={Isolation and enzymic properties of levansucrase secreted by Acetobacter diazotrophicus SRT4, a bacterium associated with sugar cane.},
  author={Lazaro E. Hernandez and Juan G. Arrieta and Carmen Men{\'e}ndez and Rafael Garc{\'i}a V{\'a}zquez and Alberto Coego and V. V. Reyes Su{\'a}rez and Guillermo Selman and Marie-Françoise Petit-Glatron and R{\'e}gis Chambert},
  journal={The Biochemical journal},
  year={1995},
  volume={309 ( Pt 1)},
  pages={113-8}
}
Acetobacter diazotrophicus, a nitrogen-fixing bacterium associated with sugar cane, secretes a levansucrase (sucrose-2,6-beta-D-fructan 6-beta-D-fructosyltransferase; EC 2.4.1.10). This enzyme is constitutively expressed and represents more than 70% of the total proteins secreted by strain SRT4. The purified protein consists of a single 58 kDa polypeptide with an isoelectric point of 5.5. Its activity is optimal at pH 5.0. It catalyses transfructosylation from sucrose to a variety of acceptors… CONTINUE READING